Calyculin A 花萼海綿體誘癌素A Ca/cAMP
簡要描述：
Calyculin A 花萼海綿體誘癌素A Ca/cAMP花萼海綿體誘癌素A 腫瘤促進(jìn)劑（CAS *01932-71-2）是一種具細(xì)胞滲透性、有效和選擇性的蛋白磷酸酶1（PP1，IC50=0.3-0.7 nM）和蛋白磷酸酶2A（PP2A，IC50=0.5-1.0 nM）抑制劑。對(duì)PP2B和PP2C的選擇性＞10,000,000倍。對(duì)酸性或堿性磷酸酶或磷酸-*蛋白磷酸
產(chǎn)品時(shí)間：2025-07-18
打印當(dāng)前頁Calyculin A 花萼海綿體誘癌素A
產(chǎn)品標(biāo)簽
Calyculin A；Okadaic acid 岡田軟海綿酸；Protein phosphatase 1 (PP1)；Protein phosphatase 2A (PP2A) ；Tumor Promoter 腫瘤促進(jìn)劑；CAS NO：101932-71-2；
產(chǎn)品信息
產(chǎn)品名稱 | 產(chǎn)品編號(hào) | CAS NO. | 規(guī)格 | 價(jià)格（元） |
Calyculin A 花萼海綿體誘癌素A | MZ2504-10UG | 101932-71-2 | 10 µg | 4588 |
Calyculin A 花萼海綿體誘癌素A | MZ2504-100UG | 101932-71-2 | 100 µg | 8498 |
產(chǎn)品描述
花萼海綿體誘癌素A（Calyculin A，CAS NO：101932-71-2）最初是從海綿Disodermia calyx中分離到的一種海洋毒素，是一種具細(xì)胞滲透性、有效和選擇性的蛋白磷酸酶1（PP1，IC50=0.3-0.7 nM）和蛋白磷酸酶2A（PP2A，IC50=0.5-1.0 nM）抑制劑。對(duì)PP2B和PP2C的選擇性＞10,000,000倍。對(duì)酸性或堿性磷酸酶或磷酸-酪氨suan蛋白磷酸酶無抑制。Calyculin A抑制平滑肌肌球蛋白B內(nèi)源性磷酸酶的活性，誘導(dǎo)肌肉纖維收縮，并伴隨細(xì)胞質(zhì)游離Ca2+水平提高。花萼海綿體誘癌素A還是一種非佛波類的腫瘤促進(jìn)劑。與小鼠皮膚上的蛋白磷酸酶結(jié)合，誘導(dǎo)鳥氨suan脫羧酶（ODC）活性，進(jìn)而促進(jìn)腫瘤生長。
產(chǎn)品特性
1） CAS NO：101932-71-2
2） 化學(xué)名：N-[(3S)-[4-(1E)-3-[(2R,3R,4R,7S,8S,9R)-2-[(1S,3S,4S,5R,7E,9E,11E,13Z)-14-Cyano-3,5-dihydroxy- 1-methoxy-4,6,8,9,13-pentamethyl-7,9,11,13-tetradecatetraenyl]-9-hydroxy-4,4,8-trimethyl-3-(phosphonooxy)-1,6-dioxaspiro[4.5]dec-7-yl]-1-propenyl]-2-oxazoly]butyl]-4-deoxy-4-(dimethylamino)-5-O-methyl-L-ribonamide
3） 分子式：C50H81N4O15P
4） 分子量：1009.17
5） 純度：≥95%
6） 外觀：透明薄膜或白色結(jié)晶性粉末
7） 溶解性：溶于DMSO（50mM）、無水乙醇、DMF、幾乎不溶于水
8） 化學(xué)結(jié)構(gòu)圖：
保存與運(yùn)輸方法
保存：-20oC避光干燥保存，至少2年有效。
運(yùn)輸：冰袋運(yùn)輸。
注意事項(xiàng)
1）本品不是臨床藥物，只能用于科研用途，不能用于診斷或臨床用途。
2）本品是一種腫瘤促進(jìn)劑，操作時(shí)做好防護(hù)措施避免任何途徑的直接接觸。
3）為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
使用方法【源自文獻(xiàn)，僅作參考】
文獻(xiàn)1，Hudák R et al. The Phosphatase Inhibitor Calyculin-A Impairs Clot Retraction, Platelet Activation, and Thrombin Generation. Biomed Res Int. 2017:9795271.PMID: 28680886 體外研究： 細(xì)胞類型（Cell type）：Platelet-rich plasma (PRP) sample 藥物配制（Preparation）：Calyculin-A (CLA) was dissolved in DMSO 實(shí)驗(yàn)方法（Assay）：PRP (110μL) was preincubated with either HEPES buffer containing 0.5% DMSO as control or the protein phosphatase inhibitor CLA (50nM), for 30 minutes at 37°C in a water bath. After preincubation, platelets were activated either by TRAP (20μM) or by thiomersal (200mM) for 15 minutes at 37°C in a water bath. Then PRP (5μL) was stained with 5μL monoclonal CD41-PE antibody and 5μL Annexin-V-FITC and Annexin-V binding buffer (1x concentrate) was added to examine the PS-expression of the platelets. |
文獻(xiàn)2，Blank T et al. The phosphoprotein DARPP-32 mediates cAMP-dependent potentiation of striatal N-methyl-D-aspartate responses. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14859-64. PMID: 9405704 體外研究： 細(xì)胞類型（Cell type）：Xenopus Oocytes 藥物配制（Preparation）：Calyculin A was prepared in 100% ethanol as a 1 mM stock solution. Immediately after dilution in distilled water to the appropriate concentration, calyculin A was microinjected into the oocytes according to the RNA injection protocol. 實(shí)驗(yàn)方法（Assay）：The NMDA-induced currents of striatal mRNA-injected oocytes were measured before and 8 min after 1-min application of 50 μM forskolin or before and 12 min after 1-min application of 10 nM PMA. The same protocol was followed after injecting oocytes with calyculin A to a final intracellular concentration of 500 nM 60–90 min before voltage-clamping. The intracellular concentration of calyculin A was calculated by assuming standard oocytes with a volume of 0.5 μl. |
文獻(xiàn)3，F(xiàn)abian L et al. Calyculin A, an enhancer of myosin, speeds up anaphase chromosome movement. Cell Chromosome. 2007 Mar 24;6:1. PMID: 17381845 體外研究： 細(xì)胞類型（Cell type）：Living crane-fly spermatocytes 藥物配制（Preparation）：Calyculin A was dissolved in DMSO as a 1mM or 50 μM stock solution. 實(shí)驗(yàn)方法（Assay）：Living crane-fly spermatocytes were perfused with insect Ringer's solution or with Calyculin A in insect Ringer's solution, at final concentrations of 0.5 μM, 100 nM, 50 nM, 20 nM, 10 nM or 5 nM, prepared from a 1 mM or a 50 μM Calyculin A stock in DMSO. Results showed that concentrations of 5 nM and 10 nM had no effect on anaphase chromosome movement, 20 nM had inconsistent effects, and 50 nM had consistent effects. Thus we used 50 nM for most of the experiments. |
相關(guān)產(chǎn)品
貨號(hào) | 名稱 | 規(guī)格 |
MZ2502-25UG | Calyculin A 花萼海綿體誘癌素A | 25 µg |
MZ2401-100UL | PMA (TPA, 5mg/ml) 佛波肉荳蔻醋酸（5mg/ml） | 100μl |
MZ2403-1MG | 4α-PMA (4α-TPA) 4α-佛波肉荳蔻醋酸 | 1mg |
— —Written/Edited by V. Shallan【版權(quán)歸MKBio懋康所有】
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